Showing the IC50 value on a graph.

**teknikscian** · 01-31-2007, 03:42 PM

Hello. Ive been lurking on here for a while and getting help with excel, now i need some answers!

Im doing a competition assay, and would like to show the IC50 value on the graph. i know how to calculate the IC50, but im not sure how to put it on my graph.

Heres an example of what i would like, a sigmoidal curve and a "box" showing my IC50 value...any suggestions?

**mrice** · 02-01-2007, 04:29 PM

Try a scatter plot using the second sub-type option.

(Or else use a Michalis - Menten double reciprocal plot to give you a straight line

)

**koh** · 04-15-2009, 09:38 PM

Hi. Im facing a same problem to calculate the IC50. Is there any formula to calculate the IC50?

My data are as below
x y
0 100
10 59.95
25 48.78
75 3.89
150 3.89
300 3.77
600 3.77
1200 3.77
2400 3.77

Do i need to change the x value to log?
PLease kindly assist me. I will appreaciate.

**vrmukeshved1** · 07-17-2010, 11:43 PM

facing a same problem to calculate the IC50. Is there any formula to calculate the IC50?

My data are as below
x y
258
50 17
75 27
100 34
250 46
500 50

Do i need to change the x value to log?
PLease kindly assist me. I will appreaciate.