Try this:
NOTE - This will will strip away the date portion of the Date-time code, so if the dates of the times are different, it will NOT calculate the true time difference between them..
Hope this helps
Edit-
the reason for the note is I suspect that is what has happened in the last row on your screen shot, the two dates are different, so it calculated the ACTUAL time difference between the 2 cells...
Which may be what you actually wanted, My formula only calculates the difference between the Times, ignoring the Dates
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